Pulse-field gel electrophoresis

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

N. B. Stafuzza, Abbey, C. A., Gill, C. A., Womack, J. E., and Amaral, M. E. J., Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library, vol. 11, pp. 3013-3019, 2012.

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome.

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