Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
“Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library”, vol. 11, pp. 3013-3019, 2012.
, River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome.