PTEN

Effect of TIMP1 transfection on PTEN expression in human kidney proximal tubular cells

J. X. Chen, Cai, G. Y., Chen, X. M., Liu, H., Chen, X., Peng, Y. M., Liu, F. Y., Li, Z., and Shi, S. Z., Effect of TIMP1 transfection on PTEN expression in human kidney proximal tubular cells, vol. 14, pp. 17373-17383, 2015.

To explore the role of metalloproteinase-1 (TIMP-1) tissue inhibitor in the mechanisms of kidney aging, we observed the effects of sense and antisense transfection of TIMP-1 and of metalloproteinase (MMP) inhibitors on phosphatase and tensin homolog (PTEN), vascular endothelial growth factor (VEGF), and Flk-1 expression in TIMP-1 transgenic human proximal tubular epithelial cells (HKCs). Transfected HKCs were co-incubated with 100 μM MMP-2 and MMP-9 inhibitor III for 24 h to affect enzyme inhibition.

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