Psathyrostachys huashanica

A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat

W. L. Du, Wang, J., Wang, L. M., Pang, Y. H., Wu, J., Zhao, J. X., Yang, Q. H., and Chen, X. H., A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat, vol. 12, pp. 4797-4806, 2013.

In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that the sequence of pHs27 had no primary sequence homology with known sequences, and Southern blotting confirmed this result.

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