Protoplast

Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21

L. Liu, Wei, Y. M., Zhou, X. W., Lin, J., Sun, X. F., and Tang, K. X., Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21, vol. 12, pp. 2913-2922, 2013.

An efficient Agrobacterium tumefaciens-mediated ge­netic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hy­gromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and opti­mized. PCR amplification and Southern hybridization were used to verify the transformation events.

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