Protocol

Comparison of methods to isolate DNA from Caesalpinia ferrea

C. C. Sousa, Gomes, S. O., Lopes, A. C. A., Gomes, R. L. F., Britto, F. B., Lima, P. S. C., and Valente, S. E. S., “Comparison of methods to isolate DNA from Caesalpinia ferrea”, vol. 13. pp. 4486-4493, 2014.

Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpinia ferrea are rare, it is important to develop and/or adapt a DNA extraction protocol that produces quality DNA samples to enable the design of strategies for the conservation of this threatened species.

Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines

E. M. Santos, Paula, J. F. R., Motta, P. M. C., Heinemann, M. B., Leite, R. C., Haddad, J. P. A., Del Puerto, H. L., and Reis, J. K. P., “Comparison of three methods of DNA extraction from peripheral blood mononuclear cells and lung fragments of equines”, vol. 9. pp. 1591-1598, 2010.

We compared three different protocols for DNA extraction from horse peripheral blood mononuclear cells (PBMC) and lung fragments, determining average final DNA concentration, purity, percentage of PCR amplification using β-actin, and cost. Thirty-four samples from PBMC, and 33 samples from lung fragments were submitted to DNA extraction by three different protocols. Protocol A consisted of a phenol-chloroform and isoamylic alcohol extraction, Protocol B used alkaline extraction with NaOH, and Protocol C used the DNAzol® reagent kit.

A simplified universal genomic DNA extraction protocol suitable for PCR

T. Y. Wang, Wang, L., Zhang, J. H., and Dong, W. H., “A simplified universal genomic DNA extraction protocol suitable for PCR”, vol. 10, pp. 519-525, 2011.

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents.

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