Proteomics

Comparative proteomic analysis of ductal and lobular invasive breast carcinoma

N. C. S. Oliveira, Gomig, T. H. B., Milioli, H. H., Cordeiro, F., Costa, G. G., Urban, C. A., Lima, R. S., Cavalli, I. J., Ribeiro, E. M. S. F., Oliveira, N. C. S., Gomig, T. H. B., Milioli, H. H., Cordeiro, F., Costa, G. G., Urban, C. A., Lima, R. S., Cavalli, I. J., Ribeiro, E. M. S. F., Oliveira, N. C. S., Gomig, T. H. B., Milioli, H. H., Cordeiro, F., Costa, G. G., Urban, C. A., Lima, R. S., Cavalli, I. J., and Ribeiro, E. M. S. F., Comparative proteomic analysis of ductal and lobular invasive breast carcinoma, vol. 15, p. -, 2016.

Breast cancer is the second most common cancer worldwide and the first among women. Invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) are the two major histological subtypes, and the clinical and molecular differences between them justify the search for new markers to distinguish them. As proteomic analysis allows for a powerful and analytical approach to identify potential biomarkers, we performed a comparative analysis of IDC and ILC samples by using two-dimensional electrophoresis and mass spectrometry.

Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans

D. R. Cavalcanti, Malafaia, C. B., Silva, T. D., Santos, B. S., Calazans, G. M. T., and Silva, M. V., Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans, vol. 14, pp. 14406-14421, 2015.

Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are fundamental steps and of great importance for the detection and identification of intra- and extracellular proteins of a proteome.

Weak cation magnetic separation technology and MALDI-TOF-MS in screening serum protein markers in primary type I osteoporosis

X. L. Shi, Li, C. W., Liang, B. C., He, K. H., and Li, X. Y., Weak cation magnetic separation technology and MALDI-TOF-MS in screening serum protein markers in primary type I osteoporosis, vol. 14, pp. 15285-15294, 2015.

We investigated weak cation magnetic separation technology and matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) in screening serum protein markers of primary type I osteoporosis. We selected 16 postmenopausal women with osteoporosis and nine postmenopausal women as controls to find a new method for screening biomarkers and establishing a diagnostic model for primary type I osteoporosis. Serum samples were obtained from controls and patients.

Physiological evaluation of the responses of Larix olgensis families to drought stress and proteomic analysis of the superior family

L. Zhang, Zhang, H. G., and Pang, Q. Y., Physiological evaluation of the responses of Larix olgensis families to drought stress and proteomic analysis of the superior family, vol. 14, pp. 15577-15586, 2015.

The conifer Larix olgensis has been analyzed to delineate physiological and proteomic changes that occur under drought stress. Studies of the deleterious effects of drought in the larch families have mainly focused on photosynthesis. In the present study, when the intensity of drought was increased, plant height was inhibited as both POD and MDA levels increased, which indicates oxidative stress.

Genes related to high temperature tolerance during maize seed germination

S. M. F. Dutra, Von Pinho, E. V. R., Santos, H. O., Lima, A. C., Von Pinho, R. G., and Carvalho, M. L. M., Genes related to high temperature tolerance during maize seed germination, vol. 14, pp. 18047-18058, 2015.

The identification of genes related to heat tolerance is fundamental for the development of high-quality seeds that are tolerant to heat stress condition. The objective of this study was to evaluate maize lineages and the gene expression involved in high temperature tolerance during germination using physiological tests, proteomics, and transcriptome analysis. Seeds from six maize lineages (30, 44, 54, 63, 64, and 91) with different levels of tolerance to high temperatures were used. Lineages 54 and 91 were observed to be more tolerant to high temperature conditions.

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