Protein transduction domain

Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury

C. L. Hou, Huang, Q., Wei, Y., Zhang, W., Mi, J. H., Ying, D. J., and Zhou, Z. H., Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury, vol. 11, pp. 1899-1908, 2012.

We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRІ recognition sites to the end of the primer, and 6xHis-Tag and NotІ recognition sites to its end.

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