Protein splicing

Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing

W. Melgaço Bezerra, Carvalho, C. Paiva da S., Moreira, Rde Azevedo, and Grangeiro, T. Barbosa, Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing, vol. 5, pp. 216-223, 2006.

During its biosynthesis in developing Canavalia brasiliensis seeds, the lectin ConBr undergoes a form of protein splicing in which the order of the N- and C-domains of the protein is reversed. To investigate whether these events can occur in other eukaryotic organisms, an expression system based on Pichia pastoris cells was established. A DNA fragment encoding prepro-ConBr was cloned into the vector pPICZB, and the recombinant plasmid was transformed in P. pastoris strain GS115. Ten clones were screened for effective recombinant protein production.

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