Protein-protein interaction

Identification of altered pathways in hypertrophic cardiomyopathy based on combined data of protein-protein interactions and molecular pathways

S. W. Zhang, Wang, J., Pan, J., Zhang, S. W., Wang, J., and Pan, J., “Identification of altered pathways in hypertrophic cardiomyopathy based on combined data of protein-protein interactions and molecular pathways”, vol. 15, p. -, 2016.

The purpose of our study was to identify molecular pathways altered during the pathogenesis of hypertrophic cardiomyopathy (HCM) based on data from the STRING protein-protein interaction (PPI) database and the REACTOME pathway database. Identification of differentially expressed genes (DEGs) was carried out, followed by construction of a targeted network and selection of hub genes in this network.

Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein

Q. J. Xiang, Zhai, J. F., Zhang, M., and Zhang, B., “Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein”, vol. 14, pp. 17834-17846, 2015.

In this study, the in vivo interaction system of oligopeptide permease (Opp) proteins was analyzed, and a high expression system of inner membrane protein OppC was constructed by flexible usage of the green fluorescent protein (GFP). The Escherichia coli OppC gene, which encodes a transmembrane component of oligopeptide transporter, was cloned into different vectors. Recombinant plasmids were transformed into different E. coli strains, and the expression conditions were optimized.

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