Protein

Proteomic and gene expression analyses during bolting-related leaf color change in Brassica rapa

Y. W. Zhang, Guo, M. H., Tang, X. B., Jin, D., Fang, Z. Y., Zhang, Y. W., Guo, M. H., Tang, X. B., Jin, D., and Fang, Z. Y., Proteomic and gene expression analyses during bolting-related leaf color change in Brassica rapa, vol. 15, p. -, 2016.

Bolting and flowering are key processes during the growth and development of Chinese cabbage (Brassica rapa L. ssp pekinensis). Understanding the molecular mechanisms underlying bolting and flowering is of significance for improving production of the vegetable. A leaf-color change from bright green to gray-green has been observed following differentiation of the flowering stem and before bolting in the vegetable, and is considered to be a signal for bolting.

Genetic diversity analysis of faba bean (Vicia faba L.) germplasms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis

W. W. Hou, Zhang, X. J., Shi, J. B., and Liu, Y. J., Genetic diversity analysis of faba bean (Vicia faba L.) germplasms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vol. 14, pp. 13945-13953, 2015.

To investigate genetic diversity and relationships of 101 faba bean (Vicia faba L.), landraces and varieties from different provinces of China and abroad were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). A total of 2625 unambiguous and stable bands from 101 germplasms were detected, and 36 different bands were classified according to the electrophoretic mobility patterns of the proteins as determined by the SDS-PAGE analysis, of which 16 were polymorphic.

Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans

D. R. Cavalcanti, Malafaia, C. B., Silva, T. D., Santos, B. S., Calazans, G. M. T., and Silva, M. V., Protein extraction method for the proteomic study of Zymomonas mobilis during production of ethanol and levans, vol. 14, pp. 14406-14421, 2015.

Zymomonas mobilis has aroused considerable interest owing to its rapid metabolism and efficiency in producing ethanol and by-products such as levans, sorbitol, and gluconic acid from simple sugars. We performed a proteomic analysis of Z. mobilis UFPEDA241 to provide a global profile of regulatory proteins. The choice of the methods of extraction and cell lysis are fundamental steps and of great importance for the detection and identification of intra- and extracellular proteins of a proteome.

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