Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana
“Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana”, vol. 14, pp. 16535-16545, 2015.
, The Arabidopsis thaliana genome encodes 56 subtilisin-like serine proteases (subtilases). In order to evaluate the protease activity of a previously uncharacterized subtilase, designated as AtSBT1.9, we cloned its full-length cDNA from A. thaliana seedlings. An AtSBT1.9 mature peptide coding sequence was inserted into the bacterial expression vector, pMAL-c2x, and the recombinant vector was transformed into Escherichia coli BL21 (DE3).