Prokaryotic expression

Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

C. Zhao, Fu, M. J., Qiu, L. H., Zhao, C., Fu, M. J., and Qiu, L. H., “Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon”, vol. 15, p. -, 2016.

Reduced reproductive performance of the black tiger shrimp (Penaeus monodon) has caused economic losses and hampered the fishing industry. Detailed investigation of the molecular mechanism by which the cell cycle is regulated in this organism is needed to understand the development and maturation of ovaries and oocytes, with a view to improving reproductive capacity. Cell cycle progression is mainly determined by cyclin-dependent kinase (CDK) and cyclin complexes, the cyclin E/CDK2 complex playing a key role in G1/S transition.

Cloning and prokaryotic expression of the porcine lipasin gene

M. M. Li, Geng, J., Guo, Y. J., Jiao, X. Q., Lu, W. F., Zhu, H. S., Wang, Y. Y., and Yang, G. Y., “Cloning and prokaryotic expression of the porcine lipasin gene”, vol. 14, pp. 14698-14705, 2015.

Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET21(b) vector and digested by the same restriction enzyme. The recombinant plasmid was transferred to Escherichia coli (BL21), and the lipasin protein was induced with isopropyl-β-D-thiogalactopyranoside.

Construction and expression of prokaryotic expression vectors fused with genes of Magnaporthe oryzae effector proteins and mCherry

Y. Q. Yang, Wang, H., Liang, M. L., Yan, J. L., Liu, L., Li, C. Y., and Yang, J., “Construction and expression of prokaryotic expression vectors fused with genes of Magnaporthe oryzae effector proteins and mCherry”, vol. 14, pp. 10827-10836, 2015.

The aim of the current study was to investigate the prokaryotic expression of the Magnaporthe oryzae effector genes BAS1 and BAS4 fused to the fluorescent protein mCherry. Based on previous polymorphic analysis of BAS1 and BAS4 in rice blast strains using PCR, blast strains containing the PCR products of BAS1 and BAS4 were selected for liquid culture for total RNA extraction.

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