Prokaryotic expression

Cloning and prokaryotic expression of the porcine lipasin gene

M. M. Li, Geng, J., Guo, Y. J., Jiao, X. Q., Lu, W. F., Zhu, H. S., Wang, Y. Y., and Yang, G. Y., Cloning and prokaryotic expression of the porcine lipasin gene, vol. 14, pp. 14698-14705, 2015.

Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET21(b) vector and digested by the same restriction enzyme. The recombinant plasmid was transferred to Escherichia coli (BL21), and the lipasin protein was induced with isopropyl-β-D-thiogalactopyranoside.

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