Principal component analysis

Use of fluorescent-based amplified fragment length polymorphism to resolve phylogenetic relationships of Colchicum species from Turkey

K. Ö. Metin, Türktaş, M., Ertuğrul, F., and Kaya, E., “Use of fluorescent-based amplified fragment length polymorphism to resolve phylogenetic relationships of Colchicum species from Turkey”, vol. 13, pp. 1480-1490, 2014.

The study of phylogenetic relationships between 14 Colchicum taxa spread throughout Turkey was performed using a fluorescent-based amplified fragment length polymorphism (AFLP) technique. Five primer pair combinations were used in AFLP reactions. The data set was analyzed statistically using the NTSYS 2.1 software, and the neighbor-joining and maximum parsimony methods were implemented to generate phylogenetic trees. These analyses clustered the samples into 3 main clades. Both the neighbor-joining and maximum parsimony analyses resulted in similar topologies.

Genetic diversity of HLA-DRB1 alleles in the Tujia population of Wufeng, Hubei Province, China

X. P. Qiu, Zhang, L., Zeng, F. Y., Wen, Y., Li, C., Qiu, L. X., Cheng, D. X., and Wu, X. X., “Genetic diversity of HLA-DRB1 alleles in the Tujia population of Wufeng, Hubei Province, China”, vol. 13, pp. 4756-4765, 2014.

We established a genetic database by investigating human leukocyte antigen (HLA)-DRB1 allelic frequencies in a disease-association study in the Tujia population in Wufang, Hubei, China. The allele frequencies of the HLA-DRB1 locus in 262 healthy, unrelated Tujia individuals living in the Wufeng region of the Hubei Province were analyzed using the Luminex HLA sequence-specific oligonucleotide method with a WAKFlow HLA typing kit. A total of 13 alleles were detected at the HLA-DRB1 locus.

Diversity of pea (Pisum sativum) accessions based on morphological data for sustainable field pea breeding in Argentina

I. Gatti, Espósito, M. A., Almirón, P., Cravero, V. P., and Cointry, E. L., “Diversity of pea (Pisum sativum) accessions based on morphological data for sustainable field pea breeding in Argentina”, vol. 10, pp. 3403-3410, 2011.

We characterized 13 accessions of dry peas of different origins from various growing regions in Argentina, based on three replications of 20 plants cultivated in 2009 and 2010 in a greenhouse, with the objective of selecting those with favorable characteristics for use in breeding programs.

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