Primer design

Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)

B. C. Kuhn, Mangolin, C. A., Souto, E. R., Vicient, C. M., Machado, M. F. P. S., Kuhn, B. C., Mangolin, C. A., Souto, E. R., Vicient, C. M., Machado, M. F. P. S., Kuhn, B. C., Mangolin, C. A., Souto, E. R., Vicient, C. M., and Machado, M. F. P. S., Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta), vol. 15, p. -, 2016.

Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-União, Olho Junto, and Tamboara, two samples per cultivar) were used to obtain IRAP and REMAP fingerprints. Twelve designed primers were amplified alone and in combinations.

Subscribe to Primer design