Porcine preadipocytes

AMFR gene silencing inhibits the differentiation of porcine preadipocytes

C. Z. Chen, Zhu, Y. N., Chai, M. L., Dai, L. S., Gao, Y., Jiang, H., Zhang, L. J., Ding, Y., Liu, S. Y., Li, Q. Y., Lu, W. F., Zhang, J. B., Chen, C. Z., Zhu, Y. N., Chai, M. L., Dai, L. S., Gao, Y., Jiang, H., Zhang, L. J., Ding, Y., Liu, S. Y., Li, Q. Y., Lu, W. F., Zhang, J. B., Chen, C. Z., Zhu, Y. N., Chai, M. L., Dai, L. S., Gao, Y., Jiang, H., Zhang, L. J., Ding, Y., Liu, S. Y., Li, Q. Y., Lu, W. F., and Zhang, J. B., AMFR gene silencing inhibits the differentiation of porcine preadipocytes, vol. 15, p. -, 2016.

Our study clarifies the role of the autocrine motility factor receptor (AMFR) gene in porcine preadipocyte differentiation. AMFR-siRNA was transfected into porcine preadipocytes and the preadipocytes were induced to differentiation. Subsequently, qRT-PCR was conducted to examine changes in mRNA expression of a series of genes in porcine preadipocytes, including AMFR, sterol-regulatory element-binding protein-1a (SREBP1a), SREBP2, insulin-induced gene 1 (Insig1), and Insig2.

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