Porcine

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3

X. Liu, Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., Wang, L. X., Liu, X., Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., and Wang, L. X., Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3, vol. 15, p. -, 2016.

The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons.

Cloning and prokaryotic expression of the porcine lipasin gene

M. M. Li, Geng, J., Guo, Y. J., Jiao, X. Q., Lu, W. F., Zhu, H. S., Wang, Y. Y., and Yang, G. Y., Cloning and prokaryotic expression of the porcine lipasin gene, vol. 14, pp. 14698-14705, 2015.

Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET21(b) vector and digested by the same restriction enzyme.

Expression and localization of cFLIP anti-apoptotic protein in the porcine corpus luteum and corpora albicans during the estrous cycle and pregnancy

H. Z. Jin, Shi, W. S., Tian, Y., Liu, Y., Jin, Y., and Manabe, N., Expression and localization of cFLIP anti-apoptotic protein in the porcine corpus luteum and corpora albicans during the estrous cycle and pregnancy, vol. 14, pp. 8262-8272, 2015.

We determined expression and localization of the anti-apoptotic cellular FLICE inhibitory protein (cFLIP) in the porcine corpora lutea (CL) and corpus albicans (CA) during estrous and preg­nancy. The CL and CA were collected at different stages of estrous to determine cFLIP immunolocalization, and mRNA and protein ex­pression.

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