Population structure

Genetic diversity and population structure of Castanopsis eyrei based on simple sequence repeat markers

L. H. Mao, Zhou, X. L., Fang, Y. M., Mao, L. H., Zhou, X. L., and Fang, Y. M., “Genetic diversity and population structure of Castanopsis eyrei based on simple sequence repeat markers”, vol. 15, p. -, 2016.

Castanopsis eyrei (Fagaceae) is one of the dominant tree species in mid-subtropical, evergreen, broad-leaved forests. We obtained 14 pairs of simple sequence repeat (SSR) primers from previous studies, which were used to analyze 90 C. eyrei individuals from three populations at different altitudes. Low heterozygosity was detected (Fis = 0.6124), and the observed heterozygosity was lower than the expected heterozygosity, possibly because of inbreeding and/or the population substructure.

Genetic diversity in fragmented populations of Populus talassica inferred from microsatellites: implications for conservation

X. H. Zhu, Cheng, S. P., Liao, T., Kang, X. Y., Zhu, X. H., Cheng, S. P., Liao, T., and Kang, X. Y., “Genetic diversity in fragmented populations of Populus talassica inferred from microsatellites: implications for conservation”, vol. 15, p. -, 2016.

Populus talassica Kom. is an ecologically important species endemic to central Asia. In China, its main distribution is restricted to the Ili region in the Xinjiang Autonomous Region. An understanding of genetic diversity and population structure is crucial for the development of a feasible conservation strategy. Twenty-six high-level simple sequence repeat (SSR) markers were screened and used to genotype 220 individuals from three native populations. A high level of genetic diversity and low population differentiation were revealed.

Development and characterization of microsatellite markers of the eastern keelback mullet (Liza affinis)

L. Liu, Gao, T. X., Han, Z. Q., Li, C. H., Sun, D. R., Song, N., Liu, L., Gao, T. X., Han, Z. Q., Li, C. H., Sun, D. R., and Song, N., “Development and characterization of microsatellite markers of the eastern keelback mullet (Liza affinis)”, vol. 15, p. -, 2016.

Twenty-four polymorphic microsatellite loci were isolated and characterized for Liza affinis using a (GT)13-enriched genomic library. The number of alleles per locus ranged from 3 to 9, with a mean number of 6.250. The observed and expected heterozygosities ranged from 0.417 to 1.000 and from 0.550 to 0.861, with an average of 0.859 and 0.779, respectively. Deviation from Hardy-Weinberg proportions was detected at three loci. Evidence of null alleles was found at two loci.

Genetic diversity and structure of the threatened species Sinopodophyllum hexandrum (Royle) Ying

W. Liu, Wang, J., Yin, D. X., Yang, M., Wang, P., Han, Q. S., Ma, Q. Q., Liu, J. J., Wang, J. X., Liu, W., Wang, J., Yin, D. X., Yang, M., Wang, P., Han, Q. S., Ma, Q. Q., Liu, J. J., and Wang, J. X., “Genetic diversity and structure of the threatened species Sinopodophyllum hexandrum (Royle) Ying”, vol. 15, p. -, 2016.

Sinopodophyllum hexandrum is an important medicinal plant that has been listed as an endangered species, making the conservation of its genetic diversity a priority. Therefore, the genetic diversity and population structure of S. hexandrum was investigated through inter-simple sequence repeat analysis of eight natural populations. Eleven selected primers generated 141 discernible fragments. The percentage of polymorphic bands was 37.59% at the species level, and 7.66-24.32% at the population level. Genetic diversity of S.

Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus

W. W. Cheng, Wang, D. Q., Wang, C. Y., Du, H., Wei, Q. W., Cheng, W. W., Wang, D. Q., Wang, C. Y., Du, H., Wei, Q. W., Cheng, W. W., Wang, D. Q., Wang, C. Y., Du, H., and Wei, Q. W., “Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus”, vol. 15, p. -, 2016.

Studies of genetic diversity and genetic population structure are critical for the conservation and management of endangered species. The Chinese sucker Myxocyprinus asiaticus is a vulnerable monotypic species in China, which is at a risk of decline owing to fluctuations in effective population size and other demographic and environmental factors. We screened 11 microsatellite loci in 214 individuals to assess genetic differentiation in both wild and cultured populations.

Isolation and characterization of microsatellite markers for Eucommia ulmoides (Eucommiaceae), an endangered tree, using next-generation sequencing

W. R. Zhang, Li, Y., Zhao, J., Wu, C. H., Ye, S., Yuan, W. J., Zhang, W. R., Li, Y., Zhao, J., Wu, C. H., Ye, S., Yuan, W. J., Zhang, W. R., Li, Y., Zhao, J., Wu, C. H., Ye, S., and Yuan, W. J., “Isolation and characterization of microsatellite markers for Eucommia ulmoides (Eucommiaceae), an endangered tree, using next-generation sequencing”, vol. 15, p. -, 2016.

Eucommia ulmoides Oliver, a single extant species of Eucommiaceae, is an endemic dioecious tree in China. The natural resources of E. ulmoides have rapidly declined in recent years because of the over-collection of its cortex. To design a suitable protection strategy, it is necessary to develop a set of molecular markers to investigate genetic diversity and population structure of E. ulmoides. Pyrosequencing of an enriched microsatellite library by Roche 454 FLX+ platform was used to isolate simple sequence repeats (SSRs) for E. ulmoides.

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