Population genetics

Geoffroy's side-necked turtle [Phrynops geoffroanus (Schweigger, 1812), Testudines: Chelidae] as a model for evolutionary ecotoxicology: relationship between environmental contamination, conditions and genetic variability

L. P. R. Venancio, Zuccari, D. A. P. C., and Bonini-Domingos, C. R., Geoffroy's side-necked turtle [Phrynops geoffroanus (Schweigger, 1812), Testudines: Chelidae] as a model for evolutionary ecotoxicology: relationship between environmental contamination, conditions and genetic variability, vol. 12. pp. 6858-6859, 2013.

The objective of this study was to evaluate the role of human activity factors, such as environmental contamination and habitat changes, as drivers for changing the physiological, biochemical, and genetic diversity of Geoffroy's side-necked turtle populations in one of the most impacted watersheds in southeastern Brazil. The impact of chemical and organic con­tamination was determined by ecotoxicological analyses to assess the action of some of the major components involved in protection against oxidative stress, phase I and II detoxifica­tion metabolism, and antioxidant capacity.

Worldwide diversity of the Y-chromosome tetra-local microsatellite DYS464

F. S. G. Kehdy and Pena, S. D. J., Worldwide diversity of the Y-chromosome tetra-local microsatellite DYS464, vol. 9, pp. 1525-1534, 2010.

Of all DNA markers on the human Y-chromosome, the tetra-local Y-linked microsatellite DYS464 is the most polymorphic. We genotyped DYS464 in 677 male samples collected worldwide, maintained in the HGDP-CEPH Human Genome Diversity Cell Line Panel. Fourteen different alleles were found, with allele lengths varying from 9 to 23 repeats. One hundred and seventy-five different genotypes were detected, of which 90 appeared to be continent-specific. The region with the highest percentage of unique genotypes was Africa.

Remarkably low genetic variation but high population differentiation in the climbing perch, Anabas testudineus (Anabantidae), based on the mtDNA control region

A. F. J. Jamsari, Muchlisin, Z. A., Musri, M., and Azizah, M. N. Siti, Remarkably low genetic variation but high population differentiation in the climbing perch, Anabas testudineus (Anabantidae), based on the mtDNA control region, vol. 9, pp. 1836-1843, 2010.

Anabas testudineus (Anabantidae) is an important food fish in Southeast Asia. We analyzed the mitochondrial DNA control region sequence data to evaluate the genetic variability and population structure of this species. Sixty specimens were collected from four populations in Sumatra and two populations in Peninsular Malaysia. We found a very low level of genetic variability, with five of the six populations exhibiting total absence of genetic variation.

Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp)

S. A. L. Garcia, Van der Lee, T. A. J., Ferreira, C. F., B. Hekkert, T. Lintel, Zapater, M. - F., Goodwin, S. B., Guzmán, M., Kema, G. H. J., and Souza, Jr., M. T., Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp), vol. 9, pp. 2207-2212, 2010.

We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most devastating pathogen attacking bananas (Musa spp). Recently, the entire genome sequence of M. fijiensis became available. We screened this database for VNTR markers. Forty-two primer pairs were selected for validation, based on repeat type and length and the number of repeat units.

Genetic analysis of 30 InDel markers for forensic use in five different Chinese populations

C. T. Li, Zhang, S. H., and Zhao, S. M., Genetic analysis of 30 InDel markers for forensic use in five different Chinese populations, vol. 10, pp. 964-979, 2011.

Allele frequencies of 30 insertion/deletion polymorphism (InDel) markers previously selected and validated for forensic purposes were assessed in 419 unrelated individuals originating from five different populations of P.R. China, including Chinese Han, Chinese Hui, Uighur, Mongolians, and Tibetans.

Isolation and characterization of polymorphic microsatellite loci in Aleurodicus dispersus (Hemiptera, Aleyrodidae)

G. - C. Ma, Wu, X., Ma, C. - L., Wu, H. - L., Hu, H. - Y., Niu, L. - M., and Fu, Y. - G., Isolation and characterization of polymorphic microsatellite loci in Aleurodicus dispersus (Hemiptera, Aleyrodidae), vol. 10. pp. 2523-2526, 2011.

Ten microsatellite markers were isolated and characterized from Aleurodicus dispersus, the spiraling whitefly, an exotic pest species that is considered to be one of the most serious agricultural pests on Hainan Island, China. The polymorphism of these loci was examined in individual whiteflies from Hainan Island and from the Canary Islands. All loci were polymorphic, with two to four alleles per locus. Mean observed and expected heterozygosity values were 0.773 and 0.585, respectively.

Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)

Y. Wang, Lu, H., Zheng, J., Long, K., Shi, Y. H., Gu, Z. F., and Wang, A. M., Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae), vol. 10, pp. 3230-3235, 2011.

The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per locus, expected and observed heterozygosities ranging from 0.68 to 0.94 and 0.56 to 0.81, respectively.

Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs

F. B. Britto, Mendes, D. S. F., Ogawa, M., Cintra, I. H. A., and Diniz, F. M., Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs, vol. 10. pp. 4084-4092, 2011.

We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were detected in the entire sample (80.8%) and within each locality (73.5-79.5%).

Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae)

S. Yang, Wang, L., Zhang, Y., Liu, X. C., Lin, H. R., and Meng, Z. N., Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae), vol. 10. pp. 4006-4011, 2011.

An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed and expected heterozygosities per locus varied from 0.387 to 1.000 and from 0.377 to 0.843, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium.

Pages

Subscribe to Population genetics