L. Zhang, Zhang, Z. H., Shen, F. J., Hou, R., Zhang, W. P., Liu, Y. L., Tu, K. Y., and Yang, A. L.
, “Identification and characterization of polymorphic microsatellite loci in the red-crowned crane”
, vol. 14, pp. 15169-15176, 2015.
We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes. The number of observed alleles ranged from 3 to 10. Observed and expected heterozygosities ranged from 0.197 to 0.935 and 0.453 to 0.887, respectively; the mean polymorphic information content was 0.663.