Polyvinylpyrrolidone

A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon

G. Meru, McDowell, D., Waters, V., Seibel, A., Davis, J., and McGregor, C., A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon, vol. 12, pp. 702-709, 2013.

Genomic tools for watermelon breeding are becoming increasingly available. A high throughput genotyping system would facilitate the use of DNA markers in marker-assisted selection. DNA extraction from leaf material requires prior seed germination and is often time-consuming and cost prohibitive. In an effort to develop a more efficient system, watermelon seeds of several genotypes and various seed sizes were sampled by removing ⅓ or ½ sections from the distal ends for DNA extraction, while germinating the remaining proximal parts of the seed.

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