Polymerase chain reaction

Association of N-acetyltransferase-2 polymorphism with an increased risk of coronary heart disease in a Chinese population

J. D. Sun, Yuan, H., Hu, H. Q., Yu, H. M., Sun, J. D., Yuan, H., Hu, H. Q., and Yu, H. M., Association of N-acetyltransferase-2 polymorphism with an increased risk of coronary heart disease in a Chinese population, vol. 15, p. -, 2016.

We investigated the possible correlations between N-acetyltransferase-2 (NAT2) gene polymorphisms and the risk of coronary heart disease (CHD). CHD patients (113) and healthy controls (118) were enrolled from the First People’s Hospital of Yuhang between January 2013 and June 2014. The patients were divided into mild CHD (N = 72) and severe CHD (N = 41) subgroups. DNA samples were extracted and the distributions of NAT2 polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus

N. Ghodsian, Akhlaghi, M., Ramachandran, V., Heidari, F., Haghvirdizadeh, P., Eshkoor, S. A., Etemad, A., Jamaluddin, J. A., and Ismail, P., Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus, vol. 14, pp. 18974-18979, 2015.

This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of the cheeks. Techniques including DNA extraction, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP) were utilized to assess biomarkers of DNA damage.

Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes

F. V. M. Rubatino, Carobin, N. V., Freitas, M. L., de Oliveira, V. T., Pietra, R. X., Oliveira, P. P. R., Bosco, A. A., and Jehee, F. S., Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes, vol. 14, pp. 7864-7872, 2015.

High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, primers that include CpG site(s) and are fully complementary to the methylated sequence have been proposed.

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