Polyclonal antibody

Antibody preparation and identification of the Cashmere goat c-kit protein in the testes

S. C. L. Wu, Luo, F. H., Kong, Q. F., and Wu, Y. J., “Antibody preparation and identification of the Cashmere goat c-kit protein in the testes”, vol. 13, pp. 4856-4866, 2014.

The c-kit protein plays a major role in the regulation of germ cell development. Its expression and distribution in rodent testes have been widely reported. However, research regarding c-kit expression in domestic animals is scarce, and the expression pattern and distribution of c-kit in germ cells have not been clearly defined. In this study, a specific antigenic region for goat c-kit was designed, and a c-kit polyclonal antibody was prepared. This antibody was then applied in a study evaluating c-kit expression in Cashmere goat tissues.

Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody

H. L. Zheng, Li, H., Sun, Y. S., Yang, Z. Y., and Yu, Q., “Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody”, vol. 13, pp. 6448-6454, 2014.

Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in Escherichia coli. Anti-PTHrP polyclonal antibody was then prepared from rabbits. Finally, the goat tissue expression profile of PTHrP was analyzed by Western blot with the anti-PTHrP polyclonal antibody.

Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)

R. A. Khan, Rashid, M., Wang, D., and Zhang, Y. L., “Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)”, vol. 12, pp. 4371-4382, 2013.

Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression vector to obtain recombinant protein in Escherichia coli DE-3 cells for the subsequent production of polyclonal antibody. New Zealand white rabbits were used for production of anti-pET-32a-H.a-ALP.

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