Plant seed expression system

Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds

N. Yao, Ai, L., Dong, Y. Y., Liu, X. M., Wang, D. Z., Wang, N., Li, X. W., Wang, F. W., Li, X. K., Li, H. Y., Jiang, C., Yao, N., Ai, L., Dong, Y. Y., Liu, X. M., Wang, D. Z., Wang, N., Li, X. W., Wang, F. W., Li, X. K., Li, H. Y., and Jiang, C., Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds, vol. 15, p. -, 2016.

Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development.

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