Plant RNA

A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization

Y. Q. Zhu, Wu, W. J., Xiao, H. W., Chen, H. B., Zheng, Y., Zhang, Y. J., .X.Wang, H., and Huang, L. Q., A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization, vol. 12, pp. 5537-5546, 2013.

A recently developed revolutionary approach to transcrip­tomics, RNA-Seq, and suppression subtractive hybridization are power­ful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general.

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