Plant improvement

RAPD analysis of the genetic diversity among accessions of Fabaceous forages (Poincianella spp) from the Caatinga

R. F. M. Mendes, Neto, R. B. Araújo, Nascimento, M. P. S. B. C., and Lima, P. S. C., “RAPD analysis of the genetic diversity among accessions of Fabaceous forages (Poincianella spp) from the Caatinga”, vol. 13, pp. 5832-5839, 2014.

Among members of the Fabaceae family, native to the Brazilian Caatinga, the species Poincianella pyramidalis and P. bracteosa exhibit particular potential as forage for cattle, sheep and goats. With the aim of establishing genetic relationships within Poincianella, random amplified polymorphic DNA analysis was performed on eight accessions of P. pyramidalis and two accessions of P.

Phenotype adaptability and stability of sugarcane genotypes in the sugarcane belt of the State of Pernambuco, Brazil

J. A. Dutra Filho, Junior, T. C., and Neto, D. E. Simões, “Phenotype adaptability and stability of sugarcane genotypes in the sugarcane belt of the State of Pernambuco, Brazil”, vol. 13, pp. 6865-6877, 2014.

We assessed the agroindustrial performance of 25 sugarcane genotypes adapted to the edaphoclimatic conditions of the State of Pernambuco, Brazil, within the microregions Mata Norte, Mata Sul, Região Central, Litoral Norte, and Litoral Sul. The variables analyzed were POL tonnage per hectare, sugarcane tonnage per hectare, fiber and total recoverable sugar tonnage per hectare, using a randomized block design with four repetitions.

Genomic in situ hybridization in plants

G. S. Silva and Souza, M. M., “Genomic in situ hybridization in plants”, vol. 12. pp. 2953-2965, 2013.

Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool has been applied to the study of hybrid lineages, genetic improvement programs, and studies of the evolution of polyploids.

Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers

A. T. B. Lima, de Souza, V. A. B., Gomes, R. L. F., and Lima, P. S. C., “Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers”, vol. 10, pp. 2893-2904, 2011.

The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in preliminary tests, and primers were selected from a set designed by the University of British Columbia on the basis of high levels of polymorphism and adequate band resolution.

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