Plant breeding

Indirect selection of industrial tomato genotypes that are resistant to spider mites (Tetranychus urticae)

J. E. Baier, Resende, J. T. V., Faria, M. V., Schwarz, K., and Meert, L., “Indirect selection of industrial tomato genotypes that are resistant to spider mites (Tetranychus urticae)”, vol. 14, pp. 244-252, 2015.

Acyl sugars present in the tomato Solanum lycopersicum ‘LA-716’ accession confer good levels of resistance to arthropod pests. The objective of the present study was to select F2 plants from the interspecific cross Solanum pennellii ‘LA-716’ x Solanum lycopersicum ‘Redenção’ to assess resistance to spider mites (Tetranychus urticae) based on the leaf acyl sugar content and repellence tests.

Identification of QTLs of resistance to white mold in common bean from multiple markers by using Bayesian analysis

L. A. C. Lara, Santos, J. B., Balestre, M., Lima, I. A., Pamplona, A. K. A., Veloso, J. S., and Silva, P. H., “Identification of QTLs of resistance to white mold in common bean from multiple markers by using Bayesian analysis”, vol. 14, pp. 1124-1135, 2015.

In this study, we identified simple sequence repeat, ampli­fied fragment length polymorphism, and sequence-related amplified poly­morphism markers linked to quantitative trait loci (QTLs) for resistance to white mold disease in common bean progenies derived from a cross between lines CNFC 9506 and RP-2, evaluated using the oxalic acid test and using Bayesian analysis. DNA was extracted from 186 F2 plants and their parental lines for molecular analysis.

Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora

C. A. F. Melo, Silva, G. S., and Souza, M. M., “Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora”, vol. 14, pp. 2176-2188, 2015.

The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133).

Soybean rust resistance sources and inheritance in the common bean (Phaseolus vulgaris L.)

T. L. P. O. Souza, Dessaune, S. N., Moreira, M. A., and Barros, E. G., “Soybean rust resistance sources and inheritance in the common bean (Phaseolus vulgaris L.)”, vol. 13, pp. 5626-5636, 2014.

Soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi, has been reported in common bean (Phaseolus vulgaris L.) cultivars and elite lines that were infected under controlled and natural field conditions in South Africa, the United States, Argentina, and Brazil. Although SBR is currently not a top priority problem for the common bean crop, many bean breeders are concerned about this disease because of the high severity and virulence diversity of P. pachyrhizi and its broad host range. In this study, a set of 44 P.

Experimental strategies in performing value for cultivation and use experiments for the tobacco crop II: dimension of the experimental network

C. E. Pulcinelli, Bruzi, A. T., Toledo, F. H. R. B., and Ramalho, M. A. P., “Experimental strategies in performing value for cultivation and use experiments for the tobacco crop II: dimension of the experimental network”, vol. 13, pp. 5541-5554, 2014.

In this study, we aimed to establish strategies for value for cultivation and use (VCU) experiments for the tobacco crop in the southern region of Brazil with respect to the number of environments used to assess tobacco lines. Trials of the Virginia (18 sites) and Burley (17 sites) varietal groups were conducted in the three states of the southern region of Brazil in the 2009-2010 crop season.

Genetic control of inflorescence in common bean

S. R. Guilherme, Ramalho, M. A. P., A. Abreu, deF. B., and Pereira, L. A., “Genetic control of inflorescence in common bean”, vol. 13, pp. 10349-10358, 2014.

The number of pods per common bean plant is a primary component of grain yield, which depends on the number of flowers produced and on the flower set. Thus, a larger number of flowers per plant would increase yield. Lines with inflorescences that had a large number of flowers compared to common bean plants now under cultivation were identified. We analyzed the genetic control of this trait and its association with grain yield. The cultivar BRSMG Talismã was crossed with 2 lines, L.59583 and L.59692, which have a large number of flowers.

Strategies in identifying individuals in a segregant population of common bean and implications of genotype x environment interaction in the success of selection

M. P. Mendes, Ramalho, M. A. P., and Abreu, A. F. B., “Strategies in identifying individuals in a segregant population of common bean and implications of genotype x environment interaction in the success of selection”, vol. 11, pp. 872-880, 2012.

The objective of this study was to compare the BLUP selection method with different selection strategies in F2:4 and assess the efficiency of this method on the early choice of the best common bean (Phaseolus vulgaris) lines. Fifty-one F2:4 progenies were produced from a cross between the CVIII8511 x RP-26 lines. A randomized block design was used with 20 replications and one-plant field plots.

Search for methylation-sensitive amplification polymorphisms in mutant figs

M. G. F. Rodrigues, Martins, A. B. G., Bertoni, B. W., Figueira, A., and Giuliatti, S., “Search for methylation-sensitive amplification polymorphisms in mutant figs”, vol. 12, pp. 2267-2280, 2013.

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amplification of polymorphic DNA and amplified fragment length polymorphism markers revealed no polymorphisms among select fig mutants that originated from gamma-irradiated buds.

Experimental strategies in carrying out VCU for tobacco crop I: plot design and size

F. H. R. B. Toledo, Ramalho, M. A. P., Pulcinelli, C. E., and Bruzi, A. T., “Experimental strategies in carrying out VCU for tobacco crop I: plot design and size”, vol. 12, pp. 3766-3774, 2013.

We aimed to establish standards for tobacco Valor de Cultivo e Uso (VCU) in Brazil. We obtained information regarding the size and design of plots of two varietal groups of tobacco (Virginia and Burley). Ten inbred lines of each varietal group were evaluated in a randomized complete block design with four replications. The plot contained 42 plants with six rows of seven columns each.

Establishment and molecular characterization of a sweet potato germplasm bank of the highlands of Paraná State, Brazil

L. K. P. Camargo, Mógor, A. F., Resende, J. T. V., and Da-Silva, P. R., “Establishment and molecular characterization of a sweet potato germplasm bank of the highlands of Paraná State, Brazil”, vol. 12, pp. 5574-5588, 2013.

The sweet potato (Ipomoea batatas L.) is a crop of great importance in developing countries, as a food staple, for animal feed, and potentially for biofuel. Development of cultivars adapted to specific regions within these countries would be useful. To start a breeding program, the first step is the establishment of a germplasm bank. We initiated a sweet potato germplasm bank with accessions collected from the highlands of Paraná State, Brazil.

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