Planarian

Molecular cloning and expression pattern of the DjStag gene in the planarian Dugesia japonica during embryonic development

Z. Q. Yuan, Zhang, J. Y., and Zhao, B. S., Molecular cloning and expression pattern of the DjStag gene in the planarian Dugesia japonica during embryonic development, vol. 13, pp. 188-197, 2014.

We examined STAG-related gene (DjStag) expression in the planarian Dugesia japonica. This species is common in Far Eastern countries. The DjStag cDNA includes 1362 bp and contains a 489-bp open reading frame corresponding to a deduced protein of 162 amino acids, with a 170-bp 5'-UTR and a 703-bp 3'-UTR. Phylogenetic analysis showed that DjStag is an STAG/STAG-like member. We examined the expression pattern of DjStag in this planarian during embryonic development by whole-mount in situ hybridization.

Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica)

Z. Q. Yuan, Zhao, B. S., and Zhang, J. Y., Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica), vol. 10, pp. 410-418, 2011.

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those from head, tail and trunk pieces. The maximum level of expression of DjStag transcripts occurred at five days after cutting. After regeneration for seven days, DjStag was weakly expressed.

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