Phalaenopsis

Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

Y. - W. Huang, Tsai, Y. - J., Cheng, T. - C., Chen, J. - J., and Chen, F. - C., Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids, vol. 13, pp. 9543-9557, 2014.

Phalaenopsis orchids have been regenerated by inducing protocorm-like bodies (PLBs) from etiolated leaf sections. However, the physiological and molecular mechanisms of secondary PLB development and subsequent proliferation have not been explored. Bisectionally cutting primary PLBs resulted in more secondary PLBs at 5 weeks, suggesting an embryogenic stem cell property imposed by wounding of primary PLB tissues.

Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis

Y. W. Huang, Tsai, Y. J., and Chen, F. C., Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis, vol. 13, pp. 10690-10703, 2014.

Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich repeat-receptor-like kinase domain.

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