PCR amplification

Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis

E. C. M. Lanes, Nick, C., Kuki, K. N., Freitas, R. D., and Motoike, S. Y., Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis, vol. 12, pp. 3905-3911, 2013.

Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethylammonium bromide method, and we evaluated the quantity, purity, and integrity of the resultant DNA. We also determined whether these procedures interfere with PCR amplification using SSR molecular markers.

Optimization of DNA extraction from seeds and fresh leaf tissues of wild marigold (Tagetes minuta) for polymerase chain reaction analysis

I. Shahzadi, Ahmed, R., Hassan, A., and Shah, M. M., Optimization of DNA extraction from seeds and fresh leaf tissues of wild marigold (Tagetes minuta) for polymerase chain reaction analysis, vol. 9, pp. 386-393, 2010.

Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be a medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties. Tagetes minuta characteristically contains high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA, causing erroneous or no PCR products. We tested and modified various standard protocols in an effort to isolate high-quality DNA from different plant tissues of T. minuta.

An inexpensive and rapid method for extracting papilionoid genomic DNA from herbarium specimens

M. Riahi, Zarre, S., Maassoumi, A. A., Attar, F., and S. Osaloo, K., An inexpensive and rapid method for extracting papilionoid genomic DNA from herbarium specimens, vol. 9. pp. 1334-1342, 2010.

Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with cetyl trimethylammonium bromide (CTAB); a third protocol used a Nucleospin Plant kit. DNA obtained from all three procedures was quantified and tested by PCR. Test results indicated the superiority of one of the CTAB protocols. We made some modifications, developing a protocol that produced high-quality DNA from all nine species.

Comparative analysis of different DNA extraction protocols in fresh and herbarium specimens of the genus Dalbergia

R. A. Ribeiro and Lovato, M. B., Comparative analysis of different DNA extraction protocols in fresh and herbarium specimens of the genus Dalbergia, vol. 6, pp. 173-187, 2007.

Five published DNA extraction protocols were compared for their ability to produce good quality DNA from fresh and herbarium leaves of several species of the genus Dalbergia. The leaves of these species contain high amounts of secondary metabolites, which make it difficult to perform a clean DNA extraction and thereby interfering with subsequent PCR amplification.

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