Construction of the intermediate vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221
“Construction of the intermediate vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221”, vol. 11, pp. 3091-3104, 2012.
, Molecular chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly expanded multiple cloning site with an adjacent imported CaMV35S promoter sequence. This vector allows controlled transformation of DNA in both Escherichia coli and Agrobacterium tumefaciens.