pBI121

Construction of the intermediate vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221

S. S. Ahmed, Gong, Z. - H., Ji, J. - J., Yin, Y. - X., Xiao, H. - J., Khan, M. A., Rehman, A., and Ahmad, I., Construction of the intermediate vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221, vol. 11, pp. 3091-3104, 2012.

Molecular chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly expanded multiple cloning site with an adjacent imported CaMV35S promoter sequence. This vector allows controlled transformation of DNA in both Escherichia coli and Agrobacterium tumefaciens.

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