P-glycoprotein

Association of MDR1 C3435T and C1236T single nucleotide polymorphisms with male factor infertility

S. E. Aydos, Karadağ, A., Özkan, T., Altınok, B., Bunsuz, M., Heidargholizadeh, S., Aydos, K., and Sunguroĝlu, A., “Association of MDR1 C3435T and C1236T single nucleotide polymorphisms with male factor infertility”, vol. 14, pp. 6330-6339, 2015.

Infertility affects 1 in 6 couples and approximately 1 in 25 men. Male factor infertility is a major cause of spermatogenic anomalies, the causes of which are largely unknown. Impaired repro­ductive functions in men might result from physiological, genetic, and/or environmental factors such as xenobiotics. The multi-drug re­sistance1 (MDR1) gene encodes a P-glycoprotein which has a role in the active transport of various substrates providing protection of somatic cells from potentially toxic substances, including xenobi­otics.

Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells

A. Rocejanasaroj, Tencomnao, T., and Sangkitikomol, W., “Thunbergia laurifolia extract minimizes the adverse effects of toxicants by regulating P-glycoprotein activity, CYP450, and lipid metabolism gene expression in HepG2 cells”, vol. 13, pp. 205-219, 2014.

Thunbergia laurifolia (TL) is widely used as an antidote in Thai traditional medicine against toxic substances such as alcohol, pesticides, arsenic, and strychnine. We found that the lyophilized form of TL in 80% ethanol possessed the antioxidant levels within the range 23,163.9 ± 1457.4 Trolox equivalents mM/kg dry mass and 899.8 ± 14.5 gallic acid equivalents mM/kg dry mass using the oxygen radical absorbance capacity assay and the Folin Ciocalteu phenol assay, respectively.

MDR1 C3435T polymorphism in Mexican patients with breast cancer

N. M. Macías-Gómez, Gutierrez-Angulo, M., Leal-Ugarte, E., Ramírez-Reyes, L., Peregrina-Sandoval, J., Meza-Espinoza, J. P., F. Solano, R., M. Ayala-Madrigal, dela Luz, and F. Telles, S., “MDR1 C3435T polymorphism in Mexican patients with breast cancer”, vol. 13, pp. 5018-5024, 2014.

We investigated whether the MDR1 C3435T polymorphism is associated with fibrocystic changes (FCC), infiltrating ductal breast cancer (IDBC), and/or clinical-pathological features of IDBC in Mexican patients. Samples from women who received surgical treatment in 2007 at the Centro Médico de Occidente (México) were included in the analysis.

Expression of multidrug resistance 1 and multidrug resistance-related protein 1 in C57BL/6 mice treated with benzene

J. - S. Huang, Zhao, M. - D., Shi, J. - M., Zhang, J. - H., Li, B., Fan, W., and Zhou, Y. - L., “Expression of multidrug resistance 1 and multidrug resistance-related protein 1 in C57BL/6 mice treated with benzene”, vol. 12, pp. 5842-5850, 2013.

ATP-binding cassette super family (ABC) proteins are considered key to oncology and pharmacology studies. We examined the effect of benzene on ABC pump protein levels in C57BL/6 mouse bone marrow mononuclear cells. After a 2-week gavage (200 mg/kg, 5 days per week), the number of peripheral leukocytes, lymphocytes and basophils dropped significantly; there was also a significant decrease in MDR1 and MRP1 gene expression. A significant reduction in expression of P-gp was found; however, there was no significant decrease in the expression of MRP1 and NF-κB p65.

Correlation of polymorphism C3435T of the MDR-1 gene and the response of primary chemotherapy in women with locally advanced breast cancer

F. F. O. Rodrigues, Santos, R. E., Melo, M. B., Silva, M. A. L. G., Oliveira, A. L., Rozenowicz, R. L., Ulson, L. B., and Aoki, T., “Correlation of polymorphism C3435T of the MDR-1 gene and the response of primary chemotherapy in women with locally advanced breast cancer”, vol. 7, pp. 177-183, 2008.

Primary chemotherapy is a useful strategy for the treatment of locally advanced breast cancer and therefore allows in vivo evaluation of the action of cytotoxic drugs and the possibility of accomplishing conservative breast surgeries, as well as the early treatment of metastasis. Mechanisms of resistance to the drugs include the action of protein associated with the efflux of drugs from the intracellular environment hindering their activity; one of the most studied proteins is P-glycoprotein codified by the MDR-1 gene.

Effects of ABCB1 3435C>T genotype on serum levels of cortisol and aldosterone in women with normal menstrual cycles

T. Nakamura, Okamura, N., Yagi, M., Omatsu, H., Yamamori, M., Kuwahara, A., Nishiguchi, K., Horinouchi, M., Okumura, K., and Sakaeda, T., “Effects of ABCB1 3435C>T genotype on serum levels of cortisol and aldosterone in women with normal menstrual cycles”, vol. 8, pp. 397-403, 2009.

ABCB1, also known as MDR1/P-glycoprotein, can transport cortisol and aldosterone. We examined the effects of ABCB1 polymorphisms on serum levels of cortisol and aldosterone among different phases of the normal menstrual cycle in 51 non-pregnant healthy Japanese female volunteers (22 ± 1 years old). The menstrual cycle was divided into three phases: premenstrual phase (14 days preceding the onset of menstruation, N = 22; menstrual phase, N = 11, and postmenstrual phase, N = 18). ABCB1 -129T>C, 1236C>T, 2677G>A/T, and 3435C>T genotypes were determined.

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