Overlap PCR

A new approach for cloning hLIF cDNA from genomic DNA isolated from the oral mucous membrane

Y. H. Cui, Zhu, G. Q., Chen, Q. J., Wang, Y. F., Yang, M. M., Song, Y. X., Wang, J. G., and Cao, B. Y., A new approach for cloning hLIF cDNA from genomic DNA isolated from the oral mucous membrane, vol. 10, pp. 3455-3462, 2011.

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the study of life science, but it is difficult to obtain by traditional reverse transcription. We employed a novel strategy to clone human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA, which was directly isolated from the mucous membrane of mouth. The hLIF sequence, which is 609 bp long and is composed of three exons, can be acquired within a few hours by amplifying each exon and splicing all of them using overlap-PCR.

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