Osteosarcoma

Single nucleotide polymorphism in the RECQL5 gene increased osteosarcoma susceptibility in a Chinese Han population

Y. Z. Dong, Huang, Y. X., and Lu, T., “Single nucleotide polymorphism in the RECQL5 gene increased osteosarcoma susceptibility in a Chinese Han population”, vol. 14, pp. 1899-1902, 2015.

In this study, we investigated the association between a RECQL genetic polymorphism and osteosarcoma in a Chinese population. We selected rs820196 in the RECQL5 gene and genotyped 185 patients with osteosarcoma and 201 age- and gender-matched non-cancer controls. We found that the CC genotype was more frequent in the osteosarcoma group compared to the control group (P = 0.011). We also found that the C allele was more common in osteosarcoma patients than that in control subjects (P = 0.004).

Effect of polymorphisms of vascular endothelial growth factor on prognosis in osteosarcoma patients

X. D. Li, Yu, D. D., Lu, J. R., Wu, C., and Jin, W. X., “Effect of polymorphisms of vascular endothelial growth factor on prognosis in osteosarcoma patients”, vol. 14, pp. 4354-4360, 2015.

We investigated the association between vascular endothelial growth factor (VEGF) gene +1612G/A, -634C/G, and +936G/C and the clinical outcome of osteosarcoma. Genomic DNA was isolated from blood samples, and 3 VEGF gene polymorphisms (+1612G/A, -634C/G, and +936G/C) were analyzed using polymerase chain reaction-restriction fragment length polymorphism. Of the 194 patients, 82 patients (42.27%) showed a good response to chemotherapy, while 73 (37.63%) died during the follow-up period.

Effect of variation of ABCB1 and GSTP1 on osteosarcoma survival after chemotherapy

J. Z. Li, Tian, Z. Q., Jiang, S. N., and Feng, T., “Effect of variation of ABCB1 and GSTP1 on osteosarcoma survival after chemotherapy”, vol. 13, pp. 3186-3192, 2014.

We conducted a comprehensive study to investigate the role of genes involved in metabolic and transport pathways in response to chemotherapy and clinical outcome of osteosarcoma patients. Genotyping of seven gene polymorphisms was performed on a 384-well plate format on the Sequenom MassARRAY platform in 162 patients with osteosarcoma. We studied the correlation of the seven gene polymorphisms with response to chemotherapy and clinical outcome of patients.

Relationship between RFC gene expression and intracellular drug concentration in methotrexate-resistant osteosarcoma cells

J. J. Wang and Li, G. J., “Relationship between RFC gene expression and intracellular drug concentration in methotrexate-resistant osteosarcoma cells”, vol. 13, pp. 5313-5321, 2014.

Osteosarcoma is a primary malignant tumor in adolescents, associated with high mortality and morbidity. The high-dose methotrexate (MTX) chemotherapy used to treat this disease may induce primary or secondary drug resistance, resulting in a reduced effect of comprehensive treatment. In this study, the relationship between reduced folate carrier (RFC) gene expression and intracellular drug concentration in MTX-resistant osteosarcoma cells (Saos-2) was investigated. MTX-resistant human osteosarcoma cells (Saos-2/MTX2.2, Saos-2/MTX4.4) were prepared.

Osteosarcoma arising from osteochondroma of the tibia: case report and cytogenetic findings

E. E. Engel, Nogueira-Barbosa, M. H., Brassesco, M. S., Silva, G. E. B., Valera, E. T., Peria, F. M., Motta, T. C., and Tone, L. G., “Osteosarcoma arising from osteochondroma of the tibia: case report and cytogenetic findings”, vol. 11. pp. 448-454, 2012.

Osteochondroma is a cartilage capped benign tumor developing mainly at the juxta-epiphyseal region of long bones. The rate of malignant transformation, mainly into chondrosarcoma, is estimated to be less than 1-3%. Transformation into osteosarcoma is very rare and has been reported only thirteen times. There is little information on treatment and outcome. We report the case of a secondary osteosarcoma arising in the left tibia of a 23-year-old male, 10 years after the initial diagnosis of osteochondroma and after two partial resections.

Construction of a human Fab phage display library from antibody repertoires of osteosarcoma patients

C. Dantas-Barbosa, Brígido, M. M., and Maranhão, A. Q., “Construction of a human Fab phage display library from antibody repertoires of osteosarcoma patients”, vol. 4, pp. 126-140, 2005.

Osteosarcoma is the commonest type of primary malignant bone tumor, frequently found in adolescents at sites of rapid bone growth. Despite current management protocols, up to half of the patients succumb to this disease. Moreover, there is no well-characterized molecular marker for diagnosis and prognosis. Since phage display methodology allows the selection of human antibody fragments with potential use in clinical applications, we applied this procedure to construct a recombinant Fab (antigen binding fragment) library from patients with osteosarcoma.

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