Osteogenesis

Effects of hypoxia on proliferation and osteogenic differentiation of periodontal ligament stem cells: an in vitro and in vivo study

Q. B. Zhang, Zhang, Z. Q., Fang, S. L., Liu, Y. R., Jiang, G., and Li, K. F., Effects of hypoxia on proliferation and osteogenic differentiation of periodontal ligament stem cells: an in vitro and in vivo study, vol. 13, pp. 10204-10214, 2014.

Changes in oxygen concentration may influence various innate characteristics of stem cells. The effects of varying oxygen concentration on human periodontal ligament stem cells (HPDLSCs) has not been explored, particularly under hypoxia-related conditions. First, HPDLSCs were cultured from the periodontium of human teeth using the outgrowth method. STRO-1 and CD146 expression of HPDLSCs was investigated by flow cytometry. To detect the multilineage differentiation capacities of HPDLSCs, osteogenic-like and adipogenic-like states were induced in cells.

Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology

S. Honsawek, Bumrungpanichthaworn, P., Thitiset, T., and Wolfinbarger, Jr., L., Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology, vol. 10, pp. 2093-2103, 2011.

Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA array technology. Osteogenic differentiation of HPO cells was determined using alkaline phosphatase assay.

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