Oryza sativa L.

Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress

G. P. Moraes, Benitez, L. C., Amaral, M. Ndo, Vighi, I. L., Auler, P. A., da Maia, L. C., Bianchi, V. J., and Braga, E. J. B., “Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress”, vol. 14, pp. 2384-2398, 2015.

To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions.

A simplified genomic DNA extraction protocol for pre-germination genotyping in rice

Y. B. Duan, Zhao, F. L., Chen, H. D., Li, H., Ni, D. H., Wei, P. C., Sheng, W., Teng, J. T., Zhang, A. M., and Xue, J. P., “A simplified genomic DNA extraction protocol for pre-germination genotyping in rice”, vol. 14, pp. 6369-6375, 2015.

Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming, requiring several transplantations of materials and sampling processes. In this study, we developed a simplified genomic DNA extraction protocol in rice by using amylase to treat half-seeds.

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