OPTN

Distribution and localization of abnormally expressed OPTN proteins in RGC5 retinal ganglion cells and their effects on subcellular morphology

Z. R. Xu, Jiang, F. G., and Chen, F., “Distribution and localization of abnormally expressed OPTN proteins in RGC5 retinal ganglion cells and their effects on subcellular morphology”, vol. 14, pp. 12093-12101, 2015.

The objectives of this study were to investigate the distributions of abnormally expressed optineurin (OPTN) proteins in retinal ganglion cells (RGC5s) of transgenic rats and their effects on subcellular morphological structures. Green fluorescent protein labeled EGFP wild-type (OPTNWT), E50K mutant type (OPTNE50K), and OPTN siRNA (si-OPTN) eukaryotic expression plasmids were constructed and transfected into RGC5s. Intracellular structures were labeled with organelle specific fluorescent dyes.

Effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5

Z. R. Xu, Jiang, F. G., and Chen, F., “Effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5”, vol. 14, pp. 9171-9180, 2015.

The OPTN gene is thought to be associated with certain types of glaucoma and the function of the protein for which it encodes, optineurin, has been extensively researched, but with contradictory results. We explored the effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5. Plasmids expressing wild-type (WT) or E50K mutant optineurin, or OPTN-specific double-hairpin small interfering RNA (si-RNA), were transfected into RGC-5 cells.

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