Northern blot

Identification of UV-B-induced microRNAs in wheat

B. Wang, Sun, Y. F., Song, N., Wang, X. J., Feng, H., Huang, L. L., and Kang, Z. S., Identification of UV-B-induced microRNAs in wheat, vol. 12, pp. 4213-4221, 2013.

MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the Northern blotting technique, we found that six miRNAs were highly responsive to UV-B stress in wheat.

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