Nitrate reductase

Characterization of novel nitrate reductase-deficient mutants for transgenic Dunaliella salina systems

L. J. Gao, Jia, Y. L., Li, S. K., and Qiu, L. L., Characterization of novel nitrate reductase-deficient mutants for transgenic Dunaliella salina systems, vol. 14, pp. 13289-13299, 2015.

The aim of the present study was to isolate and characterize novel nitrate reductase (NR)-deficient mutants, which may be useful for the transgenic manipulation of Dunaliella salina. Three NR-deficient mutants of D. salina, J-1, J-2, and J-3, were successfully isolated by screening for chlorate resistance after chemical mutagenesis with ethylnitrosourea. NR activity was not detected in the mutants and the expression of NR mRNA was significantly decreased. Growth analysis of D.

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