Characterization of 33 microsatellite markers and development of multiplex PCR for yellow-throated marten (Martes flavigula)
Development of polymorphic microsatellites for Sillago sihama based on next-generation sequencing and transferability to Sillago japonica
Novel visual system homeobox 1 gene mutations in Turkish patients with keratoconus
Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing
To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B.
Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology
Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of observed alleles per locus is 6.6 (ranging from 2 to 17).