NBS-LRR

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

S. Djebbi, Bouktila, D., Makni, H., Makni, M., and Mezghani-Khemakhem, M., Identification and characterization of novel NBS-LRR resistance gene analogues from the pea, vol. 14, pp. 6419-6428, 2015.

Pea (Pisum sativum) is one of the most cultivated le­gumes in the world, and its yield and seed quality are affected by a variety of pathogens. In plants, NBS-LRR (nucleotide binding site-leucine-rich repeat) is the main class of disease resistance genes. Using degenerate primers deduced from conserved motifs in the NBS domain of known resistance genes, we identified 10 NBS sequences in three varieties of P. sativum.

Identification of resistance gene analogs in Korean wild apple germplasm collections

D. E. Baek and Choi, C., Identification of resistance gene analogs in Korean wild apple germplasm collections, vol. 12, pp. 483-493, 2013.

Several plant disease resistance gene (R-gene) classes have been identified on the basis of specific conserved functional domains. Cloning of disease-resistance apple genes would be useful for breeding programs and for studying resistance mechanisms. We used a PCR approach with degenerate primers designed from conserved NBS-LRR (nucleotide binding site-leucine-rich repeat) regions of known R-genes to amplify and clone homologous sequences from six Korean wild apple germplasm collections and an individual plant of the Siberian wild apple, Malus baccata.

Isolation and characterization of NBS-LRR- resistance gene candidates in turmeric (Curcuma longa cv. surama)

R. K. Joshi, Mohanty, S., Subudhi, E., and Nayak, S., Isolation and characterization of NBS-LRR- resistance gene candidates in turmeric (Curcuma longa cv. surama), vol. 9, pp. 1796-1806, 2010.

Turmeric (Curcuma longa), an important asexually reproducing spice crop of the family Zingiberaceae is highly susceptible to bacterial and fungal pathogens. The identification of resistance gene analogs holds great promise for development of resistant turmeric cultivars. Degenerate primers designed based on known resistance genes (R-genes) were used in combinations to elucidate resistance gene analogs from Curcuma longa cultivar surama. The three primers resulted in amplicons with expected sizes of 450-600 bp.

Functional characterization and signal transduction ability of nucleotide-binding site-leucine-rich repeat resistance genes in plants

R. K. Joshi and Nayak, S., Functional characterization and signal transduction ability of nucleotide-binding site-leucine-rich repeat resistance genes in plants, vol. 10. pp. 2637-2652, 2011.

Pathogen infection in plants is often limited by a multifaceted defense response triggered by resistance genes. The most prevalent class of resistance proteins includes those that contain a nucleotide-binding site-leucine-rich repeat (NBS-LRR) domain. Over the past 15 years, more than 50 novel NBS-LRR class resistance genes have been isolated and characterized; they play a significant role in activating conserved defense-signaling networks.

Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

Y. Lu, Xu, W. H., Xie, Y. X., Zhang, X., Pu, J. J., Qi, Y. X., and Li, H. P., Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas, vol. 10, pp. 3098-3108, 2011.

Commercial banana varieties are highly susceptible to fungal pathogens, as well as bacterial pathogens, nematodes, viruses, and insect pests. The largest known family of plant resistance genes encodes proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for the isolation of candidate genes in banana that may be involved in plant defense.

Abundance and diversity of resistance genes in the sugarcane transcriptome revealed by in silico analysis

A. C. Wanderley-Nogueira, Soares-Cavalcanti, N. M., Morais, D. A. L., Belarmino, L. C., Barbosa-Silva, A., and Benko-Iseppon, A. M., Abundance and diversity of resistance genes in the sugarcane transcriptome revealed by in silico analysis, vol. 6, pp. 866-889, 2007.

Resistance genes (R-genes) are responsible for the first interaction of the plant with pathogens being responsible for the activation (or not) of the defense response. Despite their importance and abundance, no tools for their automatic annotation are available yet. The present study analyzed R-genes in the sugarcane expressed sequence tags database which includes 26 libraries of different tissues and development stages comprising 237,954 expressed sequence tags.

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