Nasopharyngeal carcinoma

Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis

S. M. Fu, Tu, Z. H., Deng, L. Q., Cai, J. H., Liang, Z., Lin, Z. Q., and Wang, Y. T., “Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis”, vol. 14, pp. 2537-2545, 2015.

We examined the function of survivin gene expression in patients with nasopharyngeal carcinoma (NPC), as well as small interfering RNA (siRNA) on controlling CNE-2 NPC proliferation and apoptosis. Immunohistological methods, in situ hybridization, and reverse transcription-polymerase chain reaction technique were used to detect survivin protein and mRNA expression. We designed an siRNA sequence to inhibit survivin gene expression. The MTT method was used to examine the function of siRNA on controlling cell growth and proliferation.

Correlation between ERCC1 expression and concurrent chemotherapy and radiotherapy in patients with locally advanced nasopharyngeal cancer

R. Liang, Lin, Y., Liu, Z. H., Liao, X. L., Yuan, C. L., Liao, S. N., and Li, Y. Q., “Correlation between ERCC1 expression and concurrent chemotherapy and radiotherapy in patients with locally advanced nasopharyngeal cancer”, vol. 14, pp. 5804-5811, 2015.

In this study, the expression of DNA excision repair cross-complementing gene 1 (ERCC1) in local advanced nasopharyngeal carcinoma has been correlated with the efficacy of concurrent chemoradiotherapy.

Module function and two-way clustering analysis of Epstein-Barr virus-related nasopharyngeal cancer

F. Chen, Liu, J., Fan, M., Wei, X. M., Xie, Y. L., Wang, L. H., and Yang, H., “Module function and two-way clustering analysis of Epstein-Barr virus-related nasopharyngeal cancer”, vol. 13, pp. 1823-1831, 2014.

This study sought to identify and characterize the function of genes as diagnostic markers for Epstein-Barr virus (EBV)-related nasopharyngeal cancer (NPC). The gene expression profile of GSE13597 was downloaded from the Gene Expression Omnibus database, which included 28 EBV-related NPC gene expression profile data sets, 25 disease samples, and 3 control samples. Data were pre-processed, and differentially expressed genes were screened using the R language. The co-expression coefficient was calculated to construct a co-expression network using Cytoscape.

Association of cyclin D1 and survivin expression with sensitivity to radiotherapy in patients with nasopharyngeal carcinoma

S. M. Fu, Xu, M. X., Lin, S. M., Liang, Z., and Cai, J. H., “Association of cyclin D1 and survivin expression with sensitivity to radiotherapy in patients with nasopharyngeal carcinoma”, vol. 13, pp. 3502-3509, 2014.

The association between cyclin D1 and survivin protein expressions with radiotherapy sensitivity in patients with nasopharyngeal carcinoma was investigated. Biopsy specimens of 72 patients with nasopharyngeal carcinoma were collected before the initiation of radiotherapy (49 cases were in the radiation-sensitive group and 23 cases were in the radiation-insensitive group). Conventional hematoxylin and eosin staining was used for tissue typing. The immunohistochemical SP method was used to detect cyclin D1 and survivin protein expression levels.

Association between p53 codon 72 polymorphisms and clinical outcome of nasopharyngeal carcinoma

M. L. Li, Dong, Y., Hao, Y. Z., Xu, N., Ning, F. L., Chen, S. S., and Yu, J. M., “Association between p53 codon 72 polymorphisms and clinical outcome of nasopharyngeal carcinoma”, vol. 13, pp. 10883-10890, 2014.

We conducted a cohort study to investigate whether polymorphisms in p53 at codon 72 are associated with tumor response and survival time of advanced nasopharyngeal carcinoma (NPC) patients treated with radiotherapy. The study population included 127 subjects with NPC who were enrolled at Binzhou Medical University between September 2008 and December 2009. Cox proportional hazard regression was used to assess the association between polymorphisms in the p53 gene and progression-free survival (PFS) and overall survival (OS) of NPC patients.

Sexual dimorphism of STGC3 tumor suppressor function in nasopharyngeal carcinoma CNE2 cells

Q. Qiu, Hu, B., Chen, Z. C., and He, X. S., “Sexual dimorphism of STGC3 tumor suppressor function in nasopharyngeal carcinoma CNE2 cells”, vol. 11, pp. 4585-4597, 2012.

STGC3 is a potential tumor suppressor in nasopharyngeal carcinoma. We previously found that CNE2 cells that re-expressed STGC3 formed smaller tumors in female mice than in male mice. Here, we investigated the sexual dimorphism of STGC3 as a tumor-suppressor in female and male nude mice injected subcutaneously with pcDNA3.1(+)-STGC3/CNE2 cells. ER-α was positively expressed in vitro in the CNE2 cells. The pcDNA3.1(+)-STGC3/CNE2 cell growth rate decreased after treatment with β-estradiol in vitro.

Screening and identification of the nucleic acid aptamers in nasopharyngeal carcinoma

W. - X. Chen, Zhang, K. - H., Zou, X. - S., Chen, Y. - Q., and Li, J. - G., “Screening and identification of the nucleic acid aptamers in nasopharyngeal carcinoma”, vol. 12, pp. 6850-6857, 2013.

To screen the nucleic acid aptamers of the EB virus-positive nasopharyngeal carcinoma cells, we used SELEX technology and synthesized in vitro a 78-nucleotide random DNA library. We used normal nasopharyngeal epithelial cells and EB virus-positive low differentiated nasopharyngeal carcinoma cells as target to conduct 10 cycles of screening, cloning, sequencing, and identification of the aptamers.

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