Mycoplasma hyopneumoniae

A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae

H. Z. A. Ishag, Liu, M. J., Yang, R. S., Xiong, Q. Y., Feng, Z. X., Shao, G. Q., Ishag, H. Z. A., Liu, M. J., Yang, R. S., Xiong, Q. Y., Feng, Z. X., Shao, G. Q., Ishag, H. Z. A., Liu, M. J., Yang, R. S., Xiong, Q. Y., Feng, Z. X., and Shao, G. Q., “A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae”, vol. 15, p. -, 2016.

Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that significantly affects the pig industry worldwide. Despite the availability of the whole genome sequence, studies on the pathogenesis of this organism have been limited due to the lack of a genetic manipulation system. Therefore, the aim of the current study was to generate a general GFP reporter vector based on a replicating plasmid. Here, we describe the feasibility of GFP reporter expression in M.

Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway

B. Ni, Bai, F. F., Wei, Y., Liu, M. J., Feng, Z. X., Xiong, Q. Y., Hua, L. Z., and Shao, G. Q., “Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway”, vol. 14, pp. 11429-11443, 2015.

Lipid-associated membrane proteins (LAMPs) are important in the pathogenicity of the Mycoplasma genus of bacteria. We investigated whether Mycoplasma hyopneumoniae LAMPs have pathogenic potential by inducing apoptosis in a St. Jude porcine lung epithelial cell line (SJPL). LAMPs from a pathogenic strain of M. hyopneumoniae (strain 232) were used in the research.

A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene

M. J. Liu, Du, G. M., Bai, F. F., Wu, Y. Z., Xiong, Q. Y., Feng, Z. X., Li, B., and Shao, G. Q., “A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene”, vol. 14, pp. 4677-4686, 2015.

The aim of this study was to establish a method for sensitive and rapid diagnosis of Mycoplasma hyopneumoniae in clinical specimens. To this effect, we employed three sets of primers specifically designed for amplification of nucleic acids under isothermal conditions. After optimization of reaction conditions, M. hyopneumoniae could be successfully detected at 63°C in 45 min through use of the loop-mediated isothermal amplification (LAMP) assay. A positive reaction was identified visually as white precipitate and confirmed by gel electrophoresis.

Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets

L. Z. Hua, Wu, Y. Z., Bai, F. F., William, K. K., Feng, Z. X., Liu, M. J., Yao, J. T., Zhang, X., and Shao, G. Q., “Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets”, vol. 13, pp. 5199-5206, 2014.

The Jiangquhai porcine lean strain (JQHPL) is a new pork meat-type strain that has been developed in recent years from the parent lines Duroc, Fengjing, and Jiangquhai pigs (DurocxFengjing pigxJiangquhai pig). Enzootic pneumonia (EP) in pigs induced by Mycoplasma hyopneumoniae (M. hyopneumoniae) is a chronic respiratory disease of pigs, generating high economic losses in the swine industry. Here, we investigated the degree of resistance to M.

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