Multiplex polymerase chain reaction

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites

I. G. Hamoy, Santos, E. J. M., and Santos, S. E. B., Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites, vol. 7, pp. 29-32, 2008.

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population.

Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers

A. Missiaggia and Grattapaglia, D., Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers, vol. 5, pp. 72-78, 2006.

We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer sequences of human microsatellite markers labeled with different fluorescent dyes. The robustness of the method was tested for the simultaneous detection and genetic analysis of microsatellites in a genetic mapping experiment.

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