Multiplex

Development and cross-species amplification of microsatellite loci for Puccinellia maritima, an important engineer saltmarsh species

R. Rouger, Vallejo-Marin, M., and Jump, A. S., Development and cross-species amplification of microsatellite loci for Puccinellia maritima, an important engineer saltmarsh species, vol. 13, pp. 3426-3431, 2014.

The grass Puccinellia maritima is an important saltmarsh ecosystem engineer exhibiting wide morphological variation, which is partially genetically determined. Nevertheless, nothing is known about its population genetics or how neutral genetic variation is distributed throughout its geographical range. Here, we describe 12 polymorphic microsatellites pooled into two multiplexes for this octoploid species. Assessment of 24 samples from three populations revealed 4 to 29 alleles per locus, with variation in allele presence and abundance between populations.

Optimization of a multiplex minisequencing protocol for population studies and medical genetics

C. M. B. Carvalho and Pena, S. D. J., Optimization of a multiplex minisequencing protocol for population studies and medical genetics, vol. 4, pp. 115-125, 2005.

Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research laboratories. Minisequencing meets the latter requirements. It is simple, non-radioactive and can be easily multiplexed by adding oligonucleotide tails of increasing size to the sequencing oligonucleotide primers.

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