Mud crab

First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers

H. Y. Ma, Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., Ma, L. B., Ma, H. Y., Li, S. J., Feng, N. N., Ma, C. Y., Wang, W., Chen, W., and Ma, L. B., “First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers”, vol. 15, p. -, 2016.

The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 molecular markers were mapped, including 60 microsatellites and 152 AFLP markers. The linkage groups ranged from 7 to 102.5 cM and covered 2746.4 cM in length.

Homology cloning, sequence characterization, and expression analysis of cDNA encoding electron transfer flavoprotein beta polypeptide in mud crab (Scylla paramamosain)

X. L. Hao, Yao, H. F., Cheng, Y. Z., and Wang, R. X., “Homology cloning, sequence characterization, and expression analysis of cDNA encoding electron transfer flavoprotein beta polypeptide in mud crab (Scylla paramamosain)”, vol. 11, pp. 4316-4322, 2012.

Electron transfer flavoproteins (ETFs) are αβ-heterodimers found in eukaryotic mitochondria and bacteria. Herein we report a full-length complementary DNA of a mud crab (Scylla paramamosain) ETF β subunit (Scpa-ETFB) isolated with a homology cloning strategy. The complete complementary DNA of the Scpa-ETFB contains a 17-nt 5'-untranslated region, a 765-nt open reading frame encoding 254 amino acids, and a 248-nt 3'-untranslated region. The high identity of Scpa-ETFB with ETFB in other organisms indicated that Scpa-ETFB is a new member of the ETFB family.

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