mtDNA

Ancient DNA in historical parchments - identifying a procedure for extraction and amplification of genetic material

T. Lech and Lech, T., “Ancient DNA in historical parchments - identifying a procedure for extraction and amplification of genetic material”, vol. 15, p. -, 2016.

Historical parchments in the form of documents, manuscripts, books, or letters, make up a large portion of cultural heritage collections. Their priceless historical value is associated with not only their content, but also the information hidden in the DNA deposited on them. Analyses of ancient DNA (aDNA) retrieved from parchments can be used in various investigations, including, but not limited to, studying their authentication, tracing the development of the culture, diplomacy, and technology, as well as obtaining information on the usage and domestication of animals.

Mitochondrial DNA dynamics during in vitro culture and pluripotency induction of a bovine Rho0 cell line

L. V. F. Pessôa, Bressan, F. F., Chiaratti, M. R., Pires, P. R. L., Perecin, F., Smith, L. C., and Meirelles, F. V., “Mitochondrial DNA dynamics during in vitro culture and pluripotency induction of a bovine Rho0 cell line”, vol. 14, pp. 14093-14104, 2015.

Large number of cellular changes and diseases are related to mutations in the mitochondrial DNA copy number. Cell culture in the presence of ethidium bromide is a known way of depleting mitochondrial DNA and is a useful model for studying such conditions. Interestingly, the morphology of these depleted cells resembles that of pluripotent cells, as they present larger and fragmented mitochondria with poorly developed cristae.

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