Molecular markers

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

B. S. Hsie, Brito, J. Z., Nova, M. X. Vila, Borges-Paluch, L. R., Silva, M. V., and Donato, V. M. S. T., Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers, vol. 14, pp. 17651-17659, 2015.

Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators.

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