Molecular markers

QTL mapping of soybean oil content for marker-assisted selection in plant breeding program

D. C. Leite, Pinheiro, J. B., Campos, J. B., Di Mauro, A. O., Unêda-Trevisoli, S. H., Leite, D. C., Pinheiro, J. B., Campos, J. B., Di Mauro, A. O., Unêda-Trevisoli, S. H., Leite, D. C., Pinheiro, J. B., Campos, J. B., Di Mauro, A. O., and Unêda-Trevisoli, S. H., QTL mapping of soybean oil content for marker-assisted selection in plant breeding program, vol. 15, p. -, 2016.

The present study was undertaken to detect and map the quantitative trait loci (QTL) related to soybean oil content. We used 244 progenies derived from a bi-parental cross of the Lineage 69 (from Universidade Estadual Paulista “Júlio de Mesquita Filho”/Faculdade de Ciências Agrárias e Veterinárias - Breeding Program) and Tucunaré cultivar. A total of 358 simple sequence repeat (SSR; microsatellite) markers were used to investigate the polymorphism between the parental lines, and for the polymorphic lines all the F2 individuals were tested.

Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice

Y. D. Zhang, Zheng, J., Liang, Z. K., Liang, Y. L., Peng, Z. H., and Wang, C. L., Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice, vol. 14, pp. 14882-14892, 2015.

Grain size is an important trait that directly influences the yield of rice. Validation and evaluation of grain genes is important in rice genetic studies and for breeding. In a population of 240 recombinant inbred lines (RILs) derived from a cross between an extra-large grain japonica variety TD70 and a small grain indica variety Kasalath, we mapped 19 QTLs controlling grain traits. These QTLs included six cloned grain genes, namely, GW2, GS3, qSW5, qGL3, GS5, and GW8.

Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation

L. G. Androcioli, Ruas, E. A., Rodrigues, L. A., Ruas, C. F., Perilla, H. E. R., and Ruas, P. M., Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation, vol. 14, pp. 15697-15704, 2015.

Phyla scaberrima (Verbenaceae) is a herbaceous peren­nial species that is distributed from Mexico (center of origin) to Colom­bia, growing in forest and swamp edges or grasslands from sea level up to an altitude of 1800 m. The chemical properties and uses in popular medicine have drastically affected the population size of this species. In this study, we investigated genetic variability in populations of P. scaberrima using AFLP markers.

Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers

C. A. Nogueira, Stafuzza, N. B., Ribeiro, T. P., Prado, A. D. L., Menezes, I. P. P., Peixoto, N., Gonçalves, P. J., and Almeida, L. M., Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers, vol. 14, pp. 15996-16005, 2015.

Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of their leaves and flowers.

Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

E. T. Werner, Soares, T. C. B., Gontijo, A. B. P. L., Neto, J. D. Souza, and Amaral, J. A. Tdo, Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers, vol. 14, pp. 16450-16460, 2015.

Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar ‘FMS brilhante’ using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers.

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Z. D. Zhao, Li, A. N., Wei, S. J., Wang, M. M., Li, S. J., and Zan, L. S., Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle, vol. 14, pp. 17439-17446, 2015.

Fatty acid transport protein 1 (FATP1), an integral membrane protein that facilitates long-chain fatty acid influx, is involved in the genetic network for oleic acid synthesis. The aim of this study was to examine the association of FATP1 polymorphisms with live animal meat quality traits in Chinese Qinchuan cattle. Quantitative real-time PCR analysis demonstrated that FATP1 has a broad tissue distribution in Qinchuan cattle and is highly expressed in longissimus dorsi muscle and back fat.

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