Molecular markers

Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst

M. A. Khan, Cheng, J. L., Mei, Z. Q., Wei, C. L., Fu, J. J., Khan, M. A., Cheng, J. L., Mei, Z. Q., Wei, C. L., and Fu, J. J., Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst, vol. 15, p. -, 2016.

Development of sequence-characterized amplified region (SCAR) markers from random-amplified polymorphic DNA (RAPD) fragments is a valuable molecular approach for the genetic identification of different species. By using SCAR markers, molecular analysis is reduced to a simple polymerase chain reaction (PCR) analysis using primers designed from the amplicon sequence of RAPD.

Comparative analysis of soybean genotype resistance to Heterodera glycines and Meloidogyne species via resistance gene analogs

P. M. H. Vieira, Arêdes, F. A. S., Ferreira, A., Ferreira, M. F. S., Vieira, P. M. H., Arêdes, F. A. S., Ferreira, A., and Ferreira, M. F. S., Comparative analysis of soybean genotype resistance to Heterodera glycines and Meloidogyne species via resistance gene analogs, vol. 15, p. -, 2016.

Nematodes are important pests of soybean throughout the world and cause high yield losses. As a control strategy, the identification of resistance genes is an important aim of breeding studies. Plants possess resistance genes (R), which are responsible for the recognition of pathogens and activation of the defense system. R genes and resistance gene analogs (RGAs) possess conserved domains, from which nucleotide-binding site is the most common. Using degenerate primers originating from these domains, it is possible to identify and isolate sequences of R and RGA genes.

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